chk-48 antibody  (GraphPad Software Inc)

Journal: Pathogens

Article Title: Cerebral Organoids Derived from a Parkinson’s Patient Exhibit Unique Pathogenesis from Chikungunya Virus Infection When Compared to a Non-Parkinson’s Patient

doi: 10.3390/pathogens10070913

Figure Lengend Snippet: Chikungunya infection in PD and non-PD organoids. ( A ) DAPI was used throughout to visualize nuclei, microtubule-associated protein 2 (MAP2) was used to visualize microtubules to provide a structural reference, and CHK-48 was used to target the CHIKV E2 envelope protein. Images of organoids were obtained using an Olympus Fluoview 3000 confocal microscope. Scale bar represents 500 µm. ( B ) CHIKV titer, obtained from cell culture supernatant, decreased in both Non-PD and PD organoids, eventually becoming undetectable in PD and non-PD organoids after 7 days p.i., n = 3. ( C ) CHIKV, obtained from cell culture supernatant, was undetected via RT-PCR for both organoids types by 4 days p.i., n = 3.

Article Snippet: Chikungunya E2 monoclonal antibody CHK-48 (NR-44002) was obtained from BEI resources and used to visualize CHIKV.

Techniques: Infection, Microscopy, Cell Culture, Reverse Transcription Polymerase Chain Reaction